Bioengineering Bites
Bite-size summaries of new insights and innovations from the global bioengineering research community. Articles are ten days old or less (usually just a couple of days old). Each post has a link to the original paper if you want to read more. Find me on Twitter: @ros_daw .
Saturday 19 July 2014
Further delays
Dear readers, We have had a very turbulent time in the past few months due to illness in the family and I have been unable to continue my blog. I do apologise to those who have been regularly visiting my site to see if any new updates have appeared. I hope to start again in a few weeks time. Many thanks for your patience.
Sunday 8 June 2014
Break
Dear readers - due to a serious illness in the family I will not be posting here for a while. Hopefully back up and running in a week or so. Apologies and many thanks for all your support.
Friday 6 June 2014
Cells in silica
Individual mammalian cells have been encapsulated in silica coatings using a quick and
relatively mild process carried out in cell culture medium. The coatings contain thiol groups from molecules
left over from the synthesis process offering opportunities for
post-functionalisation. Cell
proliferation was suppressed by the silica coat which also protected the cells from
the effects of trypsin and highly toxic poly (allylamine hydrochloride). Researchers suggest that modifications of the
coating could protect cells against a variety of other stressors such as heat
and UV. The approach might ultimately be useful for
applications where cells require protection and preservation such as cell-based
sensors as well as in single-cell studies.
Cytoprotective
Silica Coating of Individual Mammalian Cells Through Bioinspired Silicification,
J. Lee et al, Angewandte Chemie
International Edition; DOI: 10.1002/anie.201402280
Thursday 5 June 2014
The effect of hyperglycaemic and hyperlipidemic conditions on cardiac cells in vitro
A simple cell culture model has captured biomechanical
effects similar to those observed in myocardial tissue during the onset of diabetic
cardiomyopathy. Cardiac myocytes were co-cultured
with cardiac fibroblasts in bilayers mimicking the layered structure of the
heart and then exposed to hyperglycaemic or hyperlipidemic conditions
associated with diabetes. In both
cases, particle-tracking microrheology revealed myocyte (but not fibroblast) stiffening;
AFM measurements supported the microrheological data.
Excess fatty acid also led to increased cFOS expression – and indicator
of hypertrophy. Further experiments hinted
at a possible mediating role for reactive oxygen species but more work is
required to understand the complex mechanisms underlying the observations.
Hyperglycemic
and Hyperlipidemic Conditions Alter Cardiac Cell
Biomechanical
Properties; J. Michaelson et al; Biophysical Journal; Volume 106 June 2014
2322–2329
Wednesday 4 June 2014
Minimally-invasive device with multipoint optical stimulation for optogenetics
Researchers
have designed a minimally-invasive device that can selectively and dynamically
illuminate multiple brain regions for optogenetics applications. The device is a waveguide comprising a single
thin optical fibre with a sharp, tapered tip coated with gold (except for the
tip). Emission of desired modes of light
was permitted at specific sites along the taper by locally removing the coating
to create windows. Each window could be
addressed by adjusting the angle of the incident light on the input facet of
the fibre. In vivo proof of principle experiments demonstrated the
effectiveness of the device.
Multipoint-Emitting
Optical Fibers for Spatially Addressable In Vivo Optogenetics; F. Pisanello et al, Neuron; http://dx.doi.org/10.1016/j.neuron.2014.04.041
Tuesday 3 June 2014
Enhancing combined chemo- and radiotherapy in vivo with nanotechnology
A new targeted cancer therapy
termed quadrapeutics (after the four components of the therapy) radically
accelerated and improved the effect of combined chemotherapy and radiation in vivo.
Cancer cells self-assembled systemically administered
antibody-functionalized gold nanoparticles and drug-loaded nanocarriers into
intracellular nanoclusters via receptor-mediated endocytosis. Near-infra-red laser pulses heated the gold
nanoparticles generating vapour plasmonic nanobubbles which then exploded,
releasing the drugs from the nanocarriers into the cytoplasm. Subsequently, xray pulses were locally
amplified in the cancer cells through the emission of secondary electrons by
the gold nanoparticles in the nanoclusters.
Nanocluster size and thus effectiveness increased with the cancer
aggressiveness.
On-demand intracellular
amplification of chemoradiation with cancer-specific plasmonic nanobubbles; Ekaterina Y Lukianova-Hleb et al; Nature Medicine; doi:10.1038/nm.3484
Monday 2 June 2014
Stem cell culture
June’s
issue of Nature Materials has a focus on stem cell culture. The first of three reviews discusses how various
inherent properties of materials may be engineered to regulate stem cell
decisions, while a second focuses on the influence of the nano scale
extracellular environment on stem cell fate via integrin-matrix
interactions. The third review outlines progress
in high-throughput materials discovery of growth
substrates for large-scale human pluripotent stem cell culture. A Perspective article discusses the interplay
between soluble factors and physical microenvironment in the control of stem
cell fate. A variety of primary research
articles and supporting “News and Views” pieces are also contained in the focus. Worth a look.
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