A simple cell culture model has captured biomechanical
effects similar to those observed in myocardial tissue during the onset of diabetic
cardiomyopathy. Cardiac myocytes were co-cultured
with cardiac fibroblasts in bilayers mimicking the layered structure of the
heart and then exposed to hyperglycaemic or hyperlipidemic conditions
associated with diabetes. In both
cases, particle-tracking microrheology revealed myocyte (but not fibroblast) stiffening;
AFM measurements supported the microrheological data.
Excess fatty acid also led to increased cFOS expression – and indicator
of hypertrophy. Further experiments hinted
at a possible mediating role for reactive oxygen species but more work is
required to understand the complex mechanisms underlying the observations.
Hyperglycemic
and Hyperlipidemic Conditions Alter Cardiac Cell
Biomechanical
Properties; J. Michaelson et al; Biophysical Journal; Volume 106 June 2014
2322–2329
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